Correlation between G6PD deficiency and some hematological parameters

Abstract

The most prevalent enzyme deficiency in the world, glucose-6-phosphate dehydrogenase
deficiency (G6PD deficiency), is an inherited metabolic defect that increases the risk of
hemolytic anemia. The purpose of this study to evaluate the methods used to detect G6PD
Deficiency approved in our laboratories as the best marker to determine the deficiency of the
G6PD enzyme. Fifty samples were collected from children suffering from hemolytic anemia
had been diagnosed by G6PD deficiency. Complete blood count (CBC) had been done by
automated hematology analyzer. G6PD enzyme activity is determined using
spectrophotometry, which measures NADPH generated based on changes in the sample's
absorbance at 340 nm over time. Reticulocyte count also was detected. From total of 50 child
patients, 34 (68%) were male and 16 (32%) were Female with significant differences between
them at (p value= 0.001). The age group 0-2 years was the most prevalent group that has
G6PD deficiency 21/50 (42%). The incidence of the disease in children between the ages of
2-4, 4-6, and under 6 years were 15/50 (30%), 8/50 (16%), and 6/50 (12%) respectively. From
fifty participants in this study, 88% of them showed low hemoglobin levels, whereas, 100%
of patients have low red blood cell count. Increasing of reticulocytes has been detected in 100
% of participants. 84% of patients revealed increasing in WBC count.