Study the effect of Taraxacum officinale on the animal model of AlCl3 induce Alzheimer disease in rats.

Abstract

Background

     Alzheimer's disease is characterized by progressive deficits in memory, cognition, and behavioral impairments that ultimately lead to dementia. Two main pathogenic processes contribute to its development: oxidative stress and neuroinflammation.

Objectives:  

     This study aimed to study the effects of taraxacume officinallis (TOE) on hippocampus of the Rats and attenuate the symptom of Alzheimer disease induced by AlCl3.

Materials and Methods:

     In this case control research, 48 male albino rats in good health were split equally into six groups. The purpose of the three-month study was to assess how different therapies affected these rats. The experimental setup's specifics are as follows:

1. Healthy Control Group: This group was the initial control and was not given any medication. 2. AlCl3 Group: Each day, 10 mg/kg of AlCl3 was administered intraperitoneal
2. AlCl3 + Donepezil Group: This group was administered 10 mg/kg of AlCl3 IP daily in addition to 5 mg/kg of oral donepezil.
3. AlCl3 plus 200 mg/kg of TOE Group: The fourth group was administered an oral dose of 200 mg/kg of TOE extract in addition to 10 mg/kg of AlCl3 IP daily.
4. AlCl3 + TOE 400 mg/kg Group: The fifth group was administered 400 mg/kg of TOE extract orally daily in addition to 10 mg/kg of AlCl3 IP. 6. AlCl3 + TOE 600 mg/kg Group: The sixth group was administered an oral dose of 600 mg/kg of TOE extract daily in addition to 10 mg/kg of AlCl3 IP each day.

Neurobehavioral analysis using an eight-arm maze was performed on day 31. Following the behavioral tests, rats were killed and remove their heads, and brain tissue samples were collected and homogenized to obtain tissue supernatant. Biochemical tests were then conducted on these samples to measure the levels of interleukin 6 (IL-6) and total antioxidant capacity (TAOC).

Results:

     Comparing the effects of TOE with the aluminum chloride group, the main results indicate that rats in groups treated at different dose with TOE, spent less time finding food in the maze. This suggests that TOE effectively decrease Alzheimer's-like symptoms in rats.

Biochemical analysis further revealed that groups 5 and 6, treated with TOEshowed a significant decrease in IL-6 levels and a significant increase in TAOC compared to the AlCl3 group. These findings support the hypothesis that (TOE) has shown potential anti-Alzheimer-like activity, particularly in reversing Alzheimer's disease symptoms induced by aluminum chloride in male rats through its anti-inflammatory and antioxidant properties.

In conclusion, this study experimentally demonstrates that Taraxacum officinale exhibits potential anti-Alzheimer activity by mitigating neuro inflammation and oxidative stress.