Evaluation of the effect of valproic acid on 5-fluorouracil cytotoxicity on colon cancer cells. An in vitro study
Keywords:
colon cancer, SW480 cells, Vero cells, valproic acid, 5-fluorouracil, TNF-α, NRF2, and casp.3.Abstract
Background: The disease known as colonic rectal cancer, which only affects the colon or
rectum, is brought on by the aberrant proliferation of glandular epithelial cells in the colon.
The three primary types of CRC are colitis-associated, hereditary, and sporadic. According to
several research, family history, chronic inflammation, and dietary and lifestyle choices are
risk factors for colorectal cancer (CRC) [1]. Colorectal cancer (CRC) accounts for 10% of
cancer-related deaths, with ~1.4 million new cases and 693,900 deaths reported in 2012.
Materials and methods: colon cancer SW480 cells, Vero cells were incubated with different
concentrations of valproic acid combined with IC50 of 5-fluorouracil then MTT assay, TNF
α, NRF2, and casp.3 were assessed after 48 hours.
Results: valproic acid and 5-fluorouracil combination decreases the viability of SW480 colon
cancer cell lines in a dose-dependent way, decreasing TNF-α level, NRF2, and casp.3
concentration.
Conclusion: Our results have shown that 5-fluorouracil and valproic acid inhibit the growth
of colon cancer SW480 cell lines in a dose-dependent way and their combination induces a
more cytotoxic effect decreasing TNF-α, NRF2, and decreasing casp.3.
