Study the Neuroprotective Effects of Ethanoic extract of propolis on SH-SY5Y cell line Model of Parkinson’s Disease

Abstract

Background: Parkinson’s disease (PD) is the second most prevalent neurodegenerative
disease. There are currently more than five million PD patients worldwide. Up to date, no
curing treatment is available. The main characteristics of PD are the degeneration of
dopaminergic neurons in the substantia nigra pars compacta and the lack of dopamine in the
striatum, which result in the clinical features including tremor, bradykinesia, and muscle
stiffness.
Objective: This study aims to evaluate the neuroprotective activity of propolis ethanolic
extract (PEE) in rotenone induced Parkinson models in SH-SY5Y cells and study the
immunological and biochemical effects of (PEE) on the levels of IL-6, MDA, Dopamine and
TAOC and identify the doses that effectively decrease the levels of IL-6 and MDA in SH
SY5Y cells.
Materials and methods: SH-SY5Y cell lines were seeded in 96 tissue culture plates. All cells
were pretreated with different concentrations of propolis ethanolic extract (PEE) at serial
dilutions ranging from 31.25 to 1000 μg/ml (four replicates were used for PEE) for 2 hours at
37C then challenged with ROT (20 μg/ml), the plate was covered with a self-plastic lid. After
a 48-hour incubation period following the exposure period, the cell lines were taken for
immunoassay by ELISA method using MDA, IL-6, TAOC and dopamin Colorimetric assay.
The results: The result shows the propolis boosted the viability on SHSY5Y cell line due to
its ability to inhibit neurite outgrowth of differentiating SH-SY5Y cells. and the neurotoxic
agent rotenone shows high cytotoxic effect on SHSY5Y cell line. also (PEE) at different
concentrations show a significant decreased (p value<0.05) in MDA and IL-6 levels.
Conclusion: In conclusion, this study indicating that natural compound propolis attenuated
the neurotoxicity induced by rotenone in SH-SY5Y Parkinson’s disease cell model due to its
anti-inflammatory and antioxidant properties.