Selegiline Potentiates the Analgesic Effects of Diclofenac in Male Mice

Abstract

     Introduction: One of the most important annoying sensory and affection is the pain which associated present   or forthcoming tissue damage or referenced to such damage. Many drugs can be used for treating pain and nonsterodial anti-inflammatory drugs are the commonest agents uses for this purpose is diclofenac (Voltaren). The main actions involved for the analgesic, antipyretic, and anti-inflammatory, effect is blocking COX-1 enzyme then inhibition of prostaglandin synthesis. Selegiline is a monoamine oxidase type B that is used in advance for the management of wearing-off symptoms. One of the most important medications that is engaged in the antinociceptive mechanism which has a real function on certain CNS structure involved in the modulation of the pain like striatum, cerebral cortex, and spinal cord, is Monoamines. it was known recently a link between the intensity of the pain after operation and polymorphism of MAO-B in man, referring to possibility of potential action of monoamine oxidase-B in the perception of pain.

Materials and methods: Forty adult male   mice were used in the research. The range of their weights were 25-30 g. Estimation of the time to twisting of the tail or flicking of the tail i.e., the action referred to the tail-flick latency. The animals were grouped into 4 groups randomly. Each group had eight animals: Group one (it is a control group): The mice in this group received N.S. similar volume of the diclofenac. Group two: each mouse received diclofenac, 3 mg/kg, IP. Group three:  each mouse received 5mg/kg of selegiline, P.O. Group four:  each mouse received 5 mg/kg of selegiline, P.O. 30 minutes prior to the giving of diclofenac, 3 mg/kg, I.P.

Results: In group 2 which received diclofenac 3mg/kg, tail-flick latency significantly rised (P < 0.05). Effects of selegiline significantly (P < 0.05) prolonged after 60 minutes as compared to the baseline values of the same group and the corresponding time of control group. In the two times intervals, the tail-flick latency significantly decreased (P<0.05) as compared to similar time of diclofenac. Combination of selegiline with dilclofenac caused significantly (P < 0.05) increased.

of the time latency after thirty minutes and sixty minutes as compared to the baseline values of the same group as well as to the similar times of group 1 (control group) and selegiline group.